IdeZ IgG protease-ProtTech Inc.

  
     
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IdeZ IgG protease
Similar to IdeS protease, IdeZ specifically cleaves peptide bonds below the hinge region of IgG to form Fc and F(ab’)2 fragments. IdeZ cleaves all subclasses of human, rabbit, sheep, and monkey IgG. IdeZ also cleaves mouse IgG2a and IgG3 more efficiently than IdeS. Engineered with a C-ter strep tag; can be removed by strep tactin beads.
  • Name: IdeZ IgG protease
  • Synonyms:
  • Catalogue Number:PT-EZ-IdeZ-1
  • Application:FOR LABORATORY RESEARCH USE ONLY.
  • Price: 5000unit $320.00 25000unit $1500.00
  • Number:
  • Concentration:40unit/ul
  • Store:Long term storage at -20 C. Avoid repeated freeze-thaw cycles.

IdeZ IgG cleavage site: CPAPELLG*GPSVF

Unit definition:One unit is defined as the amount of enzyme required to digest >95% of 1 ug of human IgG in 30 minutes at 37°C.

Purity: >90% by SDS-PAGE gel

Formulation: 50 mM Phosphate buffer pH 6.6, 150 mM NaCl

Product Source: Cloned from Streptococcus equi subspecies zooepidemicus and expressed in E. coli.

Protocol for IgG cleavage

1. Substrate concentration should be around 0.5-1mg/mL.

2. Around pH 7.4, add IdeZ to substrate and incubate at 37° for at least 30 minutes.

Reference:

1. Lannergård, Jonas, and Bengt Guss. "IdeE, an IgG-endopeptidase of Streptococcus equi ssp. equi." FEMS microbiology letters 262.2 (2006): 230-235.

       

Cat No.: PT-EZ-IdeZ-1
Product Name: IdeZ IgG protease
Type: Protein
Property: Purified recombinant protein
Origin:
Source: E.coli
Application: FOR LABORATORY RESEARCH USE ONLY.
Formulation:
Purity: >90% by SDS-PAGE gel
Storage: -20 C. Avoid repeated freeze-thaw cycles.
Limitation: FOR LABORATORY RESEARCH USE ONLY.
Note: Engineered with a C-ter strep tag; can be removed by strep tactin beads.