Product Description: The capping enzyme is composed of two subunits: D1 and D12. Eukaryotic RNA must undergo a series of modifications in order to be exported from the nucleus and successfully translated into function proteins, the first mRNA modification is capping.[1][2] 5' capping is essential for mRNA stability, enhancing mRNA processing, mRNA export and translation.[2]
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Quality control: Verified by Mass Spectrometry analysis.
Product Source: Both D1 and D12 were produced in E. coli cells transformed with capping. This product is sterile and does not contain any components of animal origin.
Usage: FOR LABORATORY RESEARCH USE ONLY.
References:
Watson J (April 8, 2014). Molecular Biology of the Gene. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. pp. 429–455. ISBN 9780321762436.
Ghosh A, Lima CD (Jul–Aug 2010). "Enzymology of RNA cap synthesis". Wiley Interdisciplinary Reviews: RNA. 1 (1): 152–72. doi:10.1002/wrna.19. PMC 3962952. PMID 21956912.
| Cat No.： | PT-RNA-VCE-CAP |
| Product Name： | Capping Enzymes |
| Type： | Protein |
| Property： | |
| Origin： | |
| Source： | E.coli |
| Application： | Research in mRNA vaccine development |
| Formulation： | Sterile filtered through a 0.2 micron filter in 50% glycerol, 10 mM MES buffer at pH 6.5 |
| Purity： | >95% by SDS-PAGE gel |
| Storage： | Avoid repeated freeze-thaw cycles. 12 months at -20 C to -80 C. 1 month at 2 C to 8 C. |
| Limitation： | |
| Note： |