Product Description: The capping enzyme is composed of two subunits: D1 and D12. Eukaryotic RNA must undergo a series of modifications in order to be exported from the nucleus and successfully translated into function proteins, the first mRNA modification is capping. 5' capping is essential for mRNA stability, enhancing mRNA processing, mRNA export and translation.
Quality control: Verified by Mass Spectrometry analysis.
Product Source: Both D1 and D12 were produced in E. coli cells transformed with capping. This product is sterile and does not contain any components of animal origin.
Usage: FOR LABORATORY RESEARCH USE ONLY.
Watson J (April 8, 2014). Molecular Biology of the Gene. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. pp. 429–455. ISBN 9780321762436.
Ghosh A, Lima CD (Jul–Aug 2010). "Enzymology of RNA cap synthesis". Wiley Interdisciplinary Reviews: RNA. 1 (1): 152–72. doi:10.1002/wrna.19. PMC 3962952. PMID 21956912.
|Product Name：||Capping Enzymes|
|Application：||Research in mRNA vaccine development|
|Formulation：||Sterile filtered through a 0.2 micron filter in 50% glycerol, 10 mM MES buffer at pH 6.5|
|Purity：||>95% by SDS-PAGE gel|
|Storage：||Avoid repeated freeze-thaw cycles. 12 months at -20 C to -80 C. 1 month at 2 C to 8 C.|