IdeS IgG protease
IdeS specifically cleaves peptide bonds below the hinge region of IgG to form Fc and F(ab’)2 fragments. IdeS cleaves all subclasses of human IgG1-4, rabbit, sheep, monkey, humanized and chimeric IgGs, and Fc-fusion proteins. Engineered with a C-ter strep tag; can be removed by streptactin beads.
IdeZ IgG cleavage site: CPAPELLG*GPSVF
Unit definition:One unit is defined as the amount of enzyme required to digest >95% of 1 ug of human IgG in 30 minutes at 37°C.
Purity: >90% by SDS-PAGE gel
Formulation: 50 mM Phosphate buffer pH 6.6, 150 mM NaCl
Product Source: Cloned from Streptococcus equi subspecies zooepidemicus and expressed in E. coli.
Protocol for IgG cleavage
1. Substrate concentration should be around 0.5-1mg/mL.
2. Around pH 7.4, add IdeZ to substrate and incubate at 37° for at least 30 minutes.
Reference:
1. Lannergård, Jonas, and Bengt Guss. "IdeE, an IgG-endopeptidase of Streptococcus equi ssp. equi." FEMS microbiology letters 262.2 (2006): 230-235.
| Cat No.： | PT-EZ-IdeS-1 |
| Product Name： | IdeS IgG protease |
| Type： | Protein |
| Property： | Purified recombinant protein |
| Origin： | |
| Source： | E.coli |
| Application： | FOR LABORATORY RESEARCH USE ONLY. |
| Formulation： | |
| Purity： | >90% by SDS-PAGE gel |
| Storage： | -20 C. Avoid repeated freeze-thaw cycles. |
| Limitation： | FOR LABORATORY RESEARCH USE ONLY. |
| Note： |