Biotin ligase BirA-ProtTech Inc.

  
     
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Biotin ligase BirA
Description: Biotin ligase (BirA) ligates biotin specifically to a biotin acceptor peptide (GLNDIFEAQKIEWHE). This product comes with reaction mixture (10X concentration: 0.5M bicine buffer, pH 8.3, 100mM ATP, 100mM MgCl2, 500μM d-biotin)
  • Name: Biotin ligase BirA
  • Synonyms: BirA
  • Catalogue Number:PT-EZ-BirA
  • Application:FOR LABORATORY RESEARCH USE ONLY.
  • Price: 100 μg $200.00 1000 μg $1000.00
  • Number:
  • Concentration:0.5mg/ml
  • Store:Long term storage at 80 C. Avoid repeated freeze-thaw cycles.

Quality control assay: Biotin acceptor peptide tag fusion protein was used as a substrate. BirA activity was verified by streptavidin binding and mass spec analysis. A >95% bitinylation of the substrate was confirmed under the experimental conditions.


Protocol for biotinylation

1. BirA reaction condition: 50mM bicine buffer, pH 8.3, 10mM ATP, 10mM MgCl2, 50μM d-biotin.

2. Substrate concentration should be around 0.5-1mg/mL. Add 10-50 ug birA per mL reaction volume.

3. Incubate at room temperature for 1 hour or more. Longer reaction time is recommended at lower temperature.


Cat No.: PT-EZ-BirA
Product Name: Biotin ligase BirA
Type: Protein
Property:
Origin:
Source: E.coli
Application: FOR LABORATORY RESEARCH USE ONLY.
Formulation: 20 mM MES buffer at pH5.5, 50 mM NaCl, 10% glycerol.
Purity: 80% by SDS-PAGE gel
Storage: Long term storage at 80 C. Avoid repeated freeze-thaw cycles.
Limitation:
Note: Biotin/biotinylated proteins are more soluble in bicine buffer, therefore it is recommended. High glycerol and NaCl conc. may lower birA acitivity.